Chimeric RNA Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Li, Hui (المحرر), Elfman, Justin (المحرر)
الملخص:XII, 262 p. 56 illus., 52 illus. in color.
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اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2020.
الطبعة:1st ed. 2020.
سلاسل:Methods in Molecular Biology, 2079
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-4939-9904-0
التنسيق: الكتروني كتاب الكتروني
جدول المحتويات:
  • Prediction, Characterization, and In Silico Validation of Chimeric RNAs
  • Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope
  • Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras
  • Gene Fusion Discovery with INTEGRATE
  • Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit
  • Detection and Measurement of Chimeric RNAs by RT-PCR
  • Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells
  • RNase Protection Assay
  • Validation of Chimeric Fusion Peptides Using Proteomics Data
  • NanoString nCounter Technology: High-Throughput RNA Validation
  • Knockdown of Chimeric RNA by RNAi
  • Overexpression of Chimeric RNA by Retroviral Transduction
  • Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization
  • Confirmation of Transcriptional Read-Through Events byRT-PCR
  • Validating Gene Fusion as the Source of Chimeric RNAs
  • Chimeric RNA and Exosomes-Based Liquid Biopsy
  • SMaRT for Therapeutic Purposes
  • Case Study: Landscape of Chimeric RNAs in Bladder Cancer
  • Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.