Oligonucleotide-Based Therapies Methods and Protocols /
| مؤلف مشترك: | |
|---|---|
| مؤلفون آخرون: | , , |
| الملخص: | XI, 344 p. 69 illus., 46 illus. in color. text |
| اللغة: | الإنجليزية |
| منشور في: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
|
| الطبعة: | 1st ed. 2019. |
| سلاسل: | Methods in Molecular Biology,
2036 |
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://doi.org/10.1007/978-1-4939-9670-4 |
| التنسيق: | الكتروني كتاب الكتروني |
جدول المحتويات:
- Chemical Development of Therapeutic Oligonucleotides
- Tissue-Specific Delivery of Oligonucleotides
- Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot
- Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy
- Assessing Oligonucleotide Binding to Double-Strand DNA
- Modelling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy
- Characterization of Peptide/Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay
- Click-Shielded and Targeted Lipopolyplexes
- Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition
- Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer
- Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)
- Delivery of Antisense Oligonucleotides Mediatedby a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury
- Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy
- Injection and Infusion of Compounds to the Central Nervous System
- In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides
- Identifying Suitable Target Regions and Analyzing Off-Target Effects
- RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics
- Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS
- ADME: Assessing Pharmacokinetic-Pharmacodynamic Parameters of Oligonucleotides.