Neural Crest Cells Methods and Protocols /

Podrobná bibliografie
Korporativní autor: SpringerLink (Online service)
Další autoři: Schwarz, Quenten (Editor), Wiszniak, Sophie (Editor)
Shrnutí:X, 230 p. 36 illus. in color.
text
Jazyk:angličtina
Vydáno: New York, NY : Springer New York : Imprint: Humana, 2019.
Vydání:1st ed. 2019.
Edice:Methods in Molecular Biology, 1976
Témata:
On-line přístup:https://doi.org/10.1007/978-1-4939-9412-0
Médium: Elektronický zdroj Kniha
Obsah:
  • The neural crest: a remarkable model system for studying development and disease
  • Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example
  • Generation of neural crest progenitors from human pluripotent stem cells
  • Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling
  • Deciphering the neural crest contribution to cephalic development with avian embryos
  • In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis
  • Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants
  • Technologies for live imaging of enteric neural crest derived cells
  • Mouse embryo culture for the study of neural crest cells
  • Laser capture microdissection of murine embryonic neural crest cells
  • In vivo and in vitro quantitative analysis of neural crest cell migration
  • Proteomics in neural crest cell research
  • Neural crest methodologies in zebrafish and medaka
  • Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells
  • Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity
  • Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective.