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|a 9781493977864
|9 978-1-4939-7786-4
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|a 10.1007/978-1-4939-7786-4
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|a Photosynthesis
|h [electronic resource] :
|b Methods and Protocols /
|c edited by Sarah Covshoff.
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| 250 |
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|a 1st ed. 2018.
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| 264 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XI, 355 p. 70 illus., 47 illus. in color.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
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|a online resource
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|a text file
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 1770
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|a Survey of Tools for Measuring In Vivo Photosynthesis -- Photosynthetic Gas Exchange in Land Plants at the Leaf Level -- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants -- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS) -- Light-Response Curves in Land Plants -- Chlorophyll Fluorescence on the Fast Timescale -- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield -- Chlorophyll Fluorescence Imaging -- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry -- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting -- Extraction of Rubisco to Determine Catalytic Constants -- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts -- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues -- Evaluation of Lipids for the Study of Photosynthetic Membranes -- “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs -- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.
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| 520 |
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|a This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.
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| 650 |
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|a Botany.
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| 650 |
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|a Plant Science.
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| 700 |
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|a Covshoff, Sarah.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 710 |
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|a SpringerLink (Online service)
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|t Springer Nature eBook
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|i Printed edition:
|z 9781493977857
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| 776 |
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|i Printed edition:
|z 9781493977871
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| 776 |
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|i Printed edition:
|z 9781493992850
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| 830 |
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 1770
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-4939-7786-4
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| 912 |
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|a ZDB-2-PRO
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| 950 |
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|a Springer Protocols (Springer-12345)
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