Glioblastoma Methods and Protocols /

מידע ביבליוגרפי
מחבר תאגידי: SpringerLink (Online service)
מחברים אחרים: Placantonakis, Dimitris G. (Editor)
סיכום:XI, 241 p. 52 illus., 43 illus. in color.
text
שפה:אנגלית
יצא לאור: New York, NY : Springer New York : Imprint: Humana, 2018.
מהדורה:1st ed. 2018.
סדרה:Methods in Molecular Biology, 1741
נושאים:
גישה מקוונת:https://doi.org/10.1007/978-1-4939-7659-1
פורמט: אלקטרוני ספר
תוכן הענינים:
  • Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma
  • Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays
  • Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
  • Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens
  • Isolation of GBM Stem Cells with Flow Cytometry
  • Lentiviral Transduction of Primary Human Glioblastoma Cultures
  • Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors
  • Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF
  • Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro
  • Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry
  • Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)
  • Single-Cell RNA Sequencing of Glioblastoma Cells
  • Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures
  • Orthotopic Patient-derived Glioblastoma Xenografts in Mice
  • Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice
  • Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy
  • Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection
  • Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma
  • Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. .