Schizosaccharomyces pombe Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Summary: | XII, 244 p. 48 illus., 34 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edition: | 1st ed. 2018. |
| Series: | Methods in Molecular Biology,
1721 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-7546-4 |
| Format: | Electronic Book |
Table of Contents:
- Preparation of Solutions and Reagents
- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing
- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis
- The No-nonsens SDS-PAGE
- Crystallization of Recombinant α-actinin and Related Proteins
- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy
- Antibody Pull-down Experiments in Fission Yeast
- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe
- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast
- Random Spore Analysis in Fission Yeast
- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
- Schizosaccharomyces pombe Biotechnological Applications in Winemaking
- Schizosaccharomyces pombe Isolation Protocol. .