High-Resolution Imaging of Cellular Proteins Methods and Protocols /
| Institutionell upphovsman: | |
|---|---|
| Övriga upphovsmän: | , , |
| Sammanfattning: | XIII, 366 p. 45 illus., 21 illus. in color. text |
| Språk: | engelska |
| Publicerad: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
|
| Upplaga: | 1st ed. 2016. |
| Serie: | Methods in Molecular Biology,
1474 |
| Ämnen: | |
| Länkar: | https://doi.org/10.1007/978-1-4939-6352-2 |
| Materialtyp: | Elektronisk Bok |
Innehållsförteckning:
- Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture
- Antibody Production with Synthetic Peptides
- Production and Purification of Polyclonal Antibodies
- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab’)2 and Streptavidin
- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications
- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy
- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells
- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses
- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells
- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging
- Imaging SynapticVesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins
- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas
- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells
- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues
- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae
- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes
- Pre-Embedding Nanogold Silver and Gold Intensification
- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval
- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections
- Immuno-Gold Labelling for Scanning Electron Microscopy
- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope
- Functional Nanoscale Imaging Of Synaptic VesicleCycling With Superfast Fixation.