Advances and Clinical Practice in Pyrosequencing
| Korporativní autor: | |
|---|---|
| Další autoři: | , |
| Shrnutí: | XIV, 407 p. 156 illus., 112 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
|
| Vydání: | 1st ed. 2016. |
| Edice: | Springer Protocols Handbooks,
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| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-4939-3308-2 |
| Médium: | Elektronický zdroj Kniha |
Obsah:
- Preparation of single-stranded DNA for pyrosequencing by LATE-PCR
- A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly
- Improvement of LATE-PCR to prepare pyrosequencing template
- Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease
- Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA)
- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing
- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing
- A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit
- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample
- A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactionsdirectly from double-stranded PCR products
- Improvement of pyrosequencing to allow multiplex SNP typing in a program
- Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase
- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing
- Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing
- Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system
- Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing
- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing
- Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing
- Construction of 3-plex barcodes for differential gene-expression analysis with pyrosequencing
- Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences
- Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes
- Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for CNV analysis
- Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides
- Pyrosequencer with a senor of a photo-diode array
- Pyrosequencing on acryl-modified glass chip
- Pyrosequencing on-chip based on a gel-based solid-phase amplification
- Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR
- Comparative gene expression analysis of breast-cancer related genes by multiplex pyrosequencing coupled with sequence-barcodes
- MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer.- Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer
- Genotyping of pathogenic serotypes of S. suis with pyrosequencing
- Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing
- Detection of avian influenza A virus by pyrosequencing
- Genotyping of the alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material
- Pyrosequencing on a single cell.