Cyclin-Dependent Kinase (CDK) Inhibitors Methods and Protocols /

Bibliografiske detaljer
Institution som forfatter: SpringerLink (Online service)
Andre forfattere: Orzáez, Mar (Editor), Sancho Medina, Mónica (Editor), Pérez-Payá, Enrique (Editor)
Summary:X, 227 p. 42 illus., 22 illus. in color.
text
Sprog:engelsk
Udgivet: New York, NY : Springer New York : Imprint: Humana, 2016.
Udgivelse:1st ed. 2016.
Serier:Methods in Molecular Biology, 1336
Fag:
Online adgang:https://doi.org/10.1007/978-1-4939-2926-9
Format: Electronisk Bog
Indholdsfortegnelse:
  • Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity
  • Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
  • Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9
  • Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination
  • Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors
  •  Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors
  • Identification of Cyclin A Binders with a Fluorescent Peptide Sensor
  • Cell Synchronization Techniques to Study the Action of CDK Inhibitors
  • Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis
  • Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models
  • Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair
  • Drug Delivery Strategies of Chemical CDK Inhibitors
  • Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs
  • Evaluating Chemical CDK Inhibitors as Cell Death Inducers
  • Models for the Study of the Crosstalk between Inflammation and Cell Cycle
  • Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors.