Mouse Genetics Methods and Protocols /
| Korporativní autor: | |
|---|---|
| Další autoři: | , | 
| Shrnutí: | XIV, 475 p. 92 illus., 68 illus. in color. text  | 
| Jazyk: | angličtina | 
| Vydáno: | 
        New York, NY :
          Springer New York : Imprint: Humana,
    
        2014.
     | 
| Vydání: | 1st ed. 2014. | 
| Edice: | Methods in Molecular Biology,
              1194             | 
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-4939-1215-5 | 
| Médium: | Elektronický zdroj Kniha | 
                Obsah: 
            
                  - Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination
 - Generation of Conditional Knockout Mice
 - Generating Mouse Lines for Lineage Tracing and Knockout Studies
 - Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)
 - Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection
 - Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases
 - Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice
 - Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice
 - Generation of Genetically Engineered Mice by the piggyBac Transposon System
 - Generation and Applications of MADM-Based Mouse Genetic Mosaic System
 - Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System
 - In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis
 - Isolation and Handling of Mouse Embryonic Fibroblasts
 - Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue
 - Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells
 - Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development
 - Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System
 - Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo
 - Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts
 - Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment
 - Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty
 - Generating Double Knockout Mice to Model Genetic Intervention for DiabeticCardiomyopathy in Humans
 - Experimental Osteoarthritis Models in Mice
 - Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)
 - Mouse Models of Acute and Chronic Colitis
 - Manipulation and Assessment of Gut Microbiome for Metabolic Studies.