The Nuclear Membrane Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Hara, Yuki (المحرر), Kume, Kazunori (المحرر)
الملخص:XI, 346 p. 62 illus., 58 illus. in color.
text
اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2025.
الطبعة:1st ed. 2025.
سلاسل:Methods in Molecular Biology, 2958
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-0716-4714-1
التنسيق: الكتروني كتاب
جدول المحتويات:
  • Lipidomic profiling of isolated nuclei from mice and cultured cells
  • Profiling plant nuclear envelope composition using subtractive proteomics
  • Ultrastructural visualization of the nuclearenvelope in HeLa cells
  • Imaging nuclear envelopes using correlative AFM/fluorescence microscopy
  • Fast super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum dynamicsImmunostaining the yeast nuclear membrane for imaging by super-resolution fluorescence microscopy
  • Visualizing phosphatidic acid and diacylglycerol at the nuclear envelope in fission yeast
  • Measuring molecular mass densities at subcellular resolution using optical diffraction tomography
  • Quantification and comparison of protein distribution on nuclear membrane
  • Quantifying nuclear shape fluctuations along the cell cycle, with a focus on early mitosis
  • Micropost arrays to model ECM fiber obstacles during cell migration in confinement
  • Use of nucleoporin-conjugated beads to study the nuclear pore complex assembly on the nuclear membrane
  • T4 DNA-induced reconstruction of artificial nuclei in living mouse oocyte
  • Preparation of nucleoplasmic extract and its application in DNA end processing
  • Liposome floatation assays to study membrane interactions of nucleoporins
  • Giant unilamellar vesicles (GUVs) to study membrane interaction of nucleoporins
  • One-pot reconstitution of GPCRs into unilamellar vesicles for fluorescence-based phospholipid scramblase activity assay
  • Identifying genomic DNA sequences near the nuclear lamina using proximity biotinylation with Ascorbate Peroxidase
  • Pairing LaminB1-DamID and Immuno-3D-FISH to resolve and verify peripheral genome organization of adipogenesis
  • Measurement of spatial contact map using sequential FISH.