Synapse Development Methods and Protocols /

书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Soykan, Tolga (Editor), Poulopoulos, Alexandros (Editor)
总结:XII, 278 p. 63 illus., 59 illus. in color.
text
语言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2025.
版:2nd ed. 2025.
丛编:Methods in Molecular Biology, 2910
主题:
在线阅读:https://doi.org/10.1007/978-1-0716-4446-1
格式: 电子 电子书
书本目录:
  • Synaptogenic Assays Using Primary Neurons Cultured on Micropatterned Substrates
  • Generation of Glutamatergic Human Neurons from Induced Pluripotent Stem Cells
  • Generation of Prefrontal Cortex Neurons from Human Pluripotent Stem Cells Under Chemically Defined Conditions
  • Differentiation of Mature Dopaminergic Neurons from Human Induced Pluripotent Stem Cells
  • Prime Editing of Mouse Primary Neurons
  • Purification of Afference-Specific Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting
  • Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling
  • Monitoring Synapses via Trans-Synaptic GFP Complementation
  • Imaging Synapse Ultrastructure and Organization with STED Microscopy
  • Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microscopy Imaging of Single Stimulated Synapses
  • Image Analyses Routines for Quantification of Synaptic Density and Connectivity
  • Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus
  • Gene Knockout in the Developing Brain of Wild-Type Rodents by CRISPR In Utero Electroporation
  • Mapping Synaptic Activity at the Population and Cellular Levels with Genetically Encoded Voltage Indicators (GEVIs)
  • Visualization of Synapses in Larval Stages of Drosophila melanogaster Using the GRASP Technique
  • Sparse Labeling, Rapid Clearing, and Native Fluorescence Light Sheet Imaging in the Developing Rodent Cerebellum.