Synapse Development Methods and Protocols /
| Körperschaft: | |
|---|---|
| Weitere Verfasser: | , |
| Zusammenfassung: | XII, 278 p. 63 illus., 59 illus. in color. text |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY :
Springer US : Imprint: Humana,
2025.
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| Ausgabe: | 2nd ed. 2025. |
| Schriftenreihe: | Methods in Molecular Biology,
2910 |
| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1007/978-1-0716-4446-1 |
| Format: | Elektronisch E-Book |
Inhaltsangabe:
- Synaptogenic Assays Using Primary Neurons Cultured on Micropatterned Substrates
- Generation of Glutamatergic Human Neurons from Induced Pluripotent Stem Cells
- Generation of Prefrontal Cortex Neurons from Human Pluripotent Stem Cells Under Chemically Defined Conditions
- Differentiation of Mature Dopaminergic Neurons from Human Induced Pluripotent Stem Cells
- Prime Editing of Mouse Primary Neurons
- Purification of Afference-Specific Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting
- Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling
- Monitoring Synapses via Trans-Synaptic GFP Complementation
- Imaging Synapse Ultrastructure and Organization with STED Microscopy
- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microscopy Imaging of Single Stimulated Synapses
- Image Analyses Routines for Quantification of Synaptic Density and Connectivity
- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus
- Gene Knockout in the Developing Brain of Wild-Type Rodents by CRISPR In Utero Electroporation
- Mapping Synaptic Activity at the Population and Cellular Levels with Genetically Encoded Voltage Indicators (GEVIs)
- Visualization of Synapses in Larval Stages of Drosophila melanogaster Using the GRASP Technique
- Sparse Labeling, Rapid Clearing, and Native Fluorescence Light Sheet Imaging in the Developing Rodent Cerebellum.