Rhinovirus Methods and Protocols /

Մատենագիտական մանրամասներ
Համատեղ հեղինակ: SpringerLink (Online service)
Այլ հեղինակներ: Sajjan, Umadevi (Խմբագիր)
Ամփոփում:X, 194 p. 48 illus., 43 illus. in color.
text
Լեզու:անգլերեն
Հրապարակվել է: New York, NY : Springer US : Imprint: Humana, 2025.
Հրատարակություն:1st ed. 2025.
Շարք:Methods in Molecular Biology, 2903
Խորագրեր:
Առցանց հասանելիություն:https://doi.org/10.1007/978-1-0716-4410-2
Ձևաչափ: Էլեկտրոնային Գիրք
Բովանդակություն:
  • Detection of virus in clinical samples by PCR
  • Laboratory protocol for propagation and purification of rhinovirus A and B suitable for in vitro and in vivo infection
  • Method to generate high-titre rhinovirus C for in vitro and in vivo studies
  • Quantification of infectious rhinovirus A and B by plaque assay
  • Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR
  • Determination of binding and endocytosis of rhinovirus by flow cytometry
  • Assessment of viral replication in rhinovirus-infected airway epithelial cells
  • Analysis of adherence junctions in rhinovirus-infected airway epithelial cells
  • Culturing of airway stem cells obtained from COPD and asthma patients to assess the effects of rhinovirus infection
  • Investigation of Differentiated Nasal Epithelial Responses to Infection with Clinical Isolates of Rhinovirus A and C
  • In vivo model to assess the nasal infection by minor group rhinovirus
  • Identification and function of type 2 innate lymphoid cells in rhinovirus-infected mouse model of asthma
  • Assessment of rhinovirus infection in a mouse model of COPD
  • Infection of immature mice with rhinovirus for the study of asthma pathogenesis
  • In vitro model to test therapeutic agents against rhinovirus.