mRNA Decay Methods and Protocols /
| 団体著者: | |
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| その他の著者: | |
| 要約: | XIV, 423 p. 79 illus., 64 illus. in color. text |
| 言語: | 英語 |
| 出版事項: |
New York, NY :
Springer US : Imprint: Humana,
2025.
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| 版: | 1st ed. 2025. |
| シリーズ: | Methods in Molecular Biology,
2863 |
| 主題: | |
| オンライン･アクセス: | https://doi.org/10.1007/978-1-0716-4176-7 |
| フォーマット: | 電子媒体 図書 |
目次:
- Minimal Perturbation Analysis of mRNA Degradation Rates with Tet Off and RT qPCR
- Northern Blotting: Protocols for Radioactive and Non-Radioactive Detection of RNA
- RNA Blotting by Electrotransfer and RNA Detection
- Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Non-Radioactive Probes in Yeast
- Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate
- Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay
- Purification of Enzymatically Active Xrn1 for Removal of Non-Capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo
- Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts after In Vitro mRNA Synthesis
- Transcriptome-Wide Analysis of the 5’ Cap Status of RNA Using 5´Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing
- RNA Decay Assay: 5-Ethynyl-Uridine Labeling and Chasing
- Assessment of mRNA Decay and Calculation of Codon Occurrence to mRNA Stability Correlation Coefficients after 5-EU Metabolic Labeling
- Measurement of rRNA Synthesis and Degradation Rates by 3H-Uracil Labeling in Yeast
- A Method for Rapid Inducible RNA Decay
- reCRAC: A Stringent Method for Precise Mapping of Protein-RNA Interactions in Yeast
- Snapchat Moments of the Cell Revealed by UV-Crosslinking and RNA Co-Immunoprecipitation of Transient RNA-Protein Complexes
- Using the Tether Function Assay to Identify Potential Regulators of mRNA Translation and mRNA Decay
- Nascent and Mature RNA Profiling by Subcellular Fractionation in Human Cells
- Concurrent Profiling of Localized Transcriptome and RNA Dynamics in Neurons by Spatial SLAMseq
- Analysis of Cytoplasmic RNA Decay Targets Using the Auxin Degron System
- Detection of Nuclear RNA Decay Intermediates Using a Modified Oxford Nanopore RNA Sequencing Strategy
- Defining the True Native Ends of RNAs at Single-Molecule Level with TERA-Seq
- Global Profiling and Analysis of 5′ Monophosphorylated mRNA Decay Intermediates
- SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through Selectable Homology-Directed DNA Repair.