Hepatitis B Virus Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Guo, Haitao (المحرر), Cuconati, Andrea (المحرر)
الملخص:XII, 272 p. 46 illus., 36 illus. in color.
text
اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2024.
الطبعة:2nd ed. 2024.
سلاسل:Methods in Molecular Biology, 2837
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-0716-4027-2
التنسيق: الكتروني كتاب
جدول المحتويات:
  • Stem Cell-Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection
  • Primary Duck Hepatocyte Culture and Duck Hepatitis B Virus Infection Model
  • Hepatitis B Virus Covalently Closed Circular DNA Chromatin Immunoprecipitation Assay
  • Analysis of HBV cccDNA Minichromosome Accessibility by MNase-qPCR and High Throughput Sequencing
  • Circular Chromosome Conformation Capture (4C-Seq) Analysis of HBV-Host Chromosome DNA Interactome
  • Analysis of N6-Methyladenosine Modification of HBV RNA by Methylated RNA Immunoprecipitation
  • Structural Analysis of the Hepatitis B Virus RNA Encapsidation Signal ɛ by Selective 2′-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
  • A Particle Gel Assay for Detection of Intracellular Hepatitis B Virus Subviral Particles in Cultured Cells
  • Detection of Hepatitis B Virus Covalently Closed Circular DNA and Intermediates in Its Formation
  • Rapid and Reliable Protein-Free HBV DNA Extraction and Sensitive Branched DNA Southern Blot Assay
  • A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture
  • A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue
  • Detection and Quantification of HBV Transcripts by Full-Length 5'RACE-PCR
  • One-Step Reverse Transcriptase qPCR Method for Serum Hepatitis B Virus RNA Quantification
  • Method for Quantitative HDV RNA Detection: I, Manual Workflow (Serum and Liver Tissue) and II, Fully Automated High Throughput Workflow for Diagnostic Use
  • The Cre/loxP-Based Recombinant HBV cccDNA System In Vitro and In Vivo
  • Modeling HBV Infection and Therapy in Immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (FAH) Knockout Mice with Human Chimeric Liver
  • Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication
  • Peptide-Loaded HLA Class I Tetramer-Associated Magnetic Bead-Based Enrichment of HBV-Specific CD8+ T Cells
  • Optimized Ex Vivo Fluorospot Assay to Measure Multi-Functional HBV-Specific T Cell Frequencies in Chronic Hepatitis B Patients
  • Ex Vivo Fluorescent Labeling of HBV-Specific B Cells in Chronic Hepatitis B Patients
  • In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV Ribonuclease H Inhibitors.