Bacterial Chromatin Methods and Protocols /
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| Other Authors: | |
| Summary: | XIV, 658 p. 197 illus., 152 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2024.
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| Edition: | 2nd ed. 2024. |
| Series: | Methods in Molecular Biology,
2819 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-3930-6 |
| Format: | Electronic Book |
Table of Contents:
- Shedding light on bacterial chromosome structure: exploring the significance of 3C-based approaches
- Circuit topology analysis of single cell Hi-C data
- GeF-seq: a simple procedure for base pair resolution ChIP-seq
- ChIP-qPCR of FLAG-tagged proteins in bacteria
- Genomic SELEX screening of regulatory targets of transcription factors
- High-resolution characterization of DNA/protein complexes in living bacteria
- High throughput mapping of bacterial chromosomal conformations using massively multiplexed Mu transposition
- In vivo genomic supercoiling mapping using Psora-seq
- Modular assembly of synthetic secondary chromosomes
- Visualization, quantification, and statistical evaluation of dynamics for DNA-binding proteins in bacteria by Single molecule tracking
- Genetic approaches to study the interplay between transcription and nucleoid-associated proteins in Escherichia coli
- Genetic engineering of Bacillus subtilis using competence-induced homologous recombination techniques
- Atomic force microscopy imaging and analysis of prokaryotic genome organization
- Atomic force microscopy characterisation of reconstituted protein-DNA complexes
- Approaches for determining DNA persistence length using Atomic Force Microscopy
- Dynamic light scattering of DNA-ligand complexes
- Microscale thermophoresis analysis of chromatin interactions
- In vitro transcription assay to quantify effects of H-NS filaments on RNA chain elongation by RNA polymerase
- Methods to quantitatively measure topological changes induced by DNA binding proteins in vivo and in vitro
- Quantitative determination of DNA bridging efficiency of chromatin proteins
- Measuring Partition Coefficients of in vitro Biomolecular Condensates using Fluorescence Correlation Spectroscopy
- Tethered Particle Motion analysis of the DNA binding properties of architectural proteins
- Quantitation of DNA binding affinity using Tethered Particle Motion
- Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers
- Approaches to the full and partial chemical synthesis of proteins
- Deciphering Sequence-Specific DNA Binding by H-NS using Molecular Simulation
- Molecular dynamics simulations of a feather-boa model of a bacterial chromosome
- Replicating Chromosomes in Whole-Cell Models of Bacteria.