HIV Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Prasad, Vinayaka R. (Editor), Kalpana, Ganjam V. (Editor)
Summary:XIV, 358 p. 67 illus., 64 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2024.
Edition:4th ed. 2024.
Series:Methods in Molecular Biology, 2807
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-3862-0
Format: Electronic Book
Table of Contents:
  • Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells
  • Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcription
  • RNA-FISH for HIV Transcription/Localization Analysis
  • Single-Cell and Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize Reactivation of Latent HIV-1
  • Visualizing HIV-1 Assembly at the T Cell Plasma Membrane using Single Molecule Localization: TIRF Microscopy
  • Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopy
  • Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues
  • Characterization of Nuclear HIV-Induced Membraneless Organelles through Fluorescence Microscopy
  • Detection of CPSF6 in Biomolecular Condensates as a Reporter of Nuclear Import
  • Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay
  • Biochemical Detection of Capsid in the Nucleus During HIV-1 Infection
  • Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones and Specific Viral Proteins
  • Measurement of HIV Rev-Rev Response Element Functional Activity
  • Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA
  • Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencing
  • Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells
  • Proximity Ligation Assay to Detect the Proximity between Host Proteins and Viral Proteins of HIV-1
  • Modeling HIV-1 Infection in CNS via Infected Monocytes using Immunocompetent Brain Organoids
  • An In Vitro Model of Blood Brain Barrier for Studies on HIV Neuroinflammation and CNS Antibody Penetration
  • Generation of Anti-HIV CAR-T Cells for Preclinical Research
  • Highly Sensitive Analysis of Cervical Mucosal HIV-1 Infection using Reporter Viruses Expressing Secreted Nanoluciferase
  • Multiparameter Flow Cytometry Monitoring of T Cell Responses
  • An Ultrasensitive p24 Assay to Measure HIV-1 in Diverse Biological Matrixes.