Mammalian Synthetic Systems

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ceroni, Francesca (Editor), Polizzi, Karen (Editor)
Summary:XI, 316 p. 88 illus.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2024.
Edition:1st ed. 2024.
Series:Methods in Molecular Biology, 2774
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-3718-0
Format: Electronic Book
Table of Contents:
  • A directed evolution protocol for engineering minimal transcription factors, based on CIS display
  • Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems
  • Coiled-coiled interaction toolbox for engineering mammalian cells
  • A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces
  • Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells
  • Mechanistic model-driven biodesign in mammalian synthetic biology
  • Realizing Antithetic Integral Feedback Control in Mammalian Cells
  • A computational modeling approach for the design of genetic control systems that respond to transcriptional activity
  • Flux Balance Analysis of Mammalian Cell Systems
  • Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing
  • Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins
  • Multiplexed transactivation of mammalian cells using dFnCas12a-VPR
  • Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems
  • Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
  • Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
  • Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
  • High-throughput spectroscopic analysis of mRNA capping level
  • In vitro generation of megakaryocytes from engineered mouse embryonic stem cells
  • Preparation of chromobodies for the detection of cell surface epitopes.