Hypoxia Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Gilkes, Daniele M. (Editor)
Summary:XI, 266 p. 69 illus., 59 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2024.
Edition:1st ed. 2024.
Series:Methods in Molecular Biology, 2755
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-3633-6
Format: Electronic Book
Table of Contents:
  • Genetically Encoded Reporters to Monitor Hypoxia
  • Detection of Hypoxia in 2D and 3D Cell Culture Systems using Genetically Encoded Fluorescent Hypoxia Sensors
  • Mapping the Fate of Hypoxic Cells using an Irreversible Fluorescent Switch
  • Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xenografted Tumor Imaging in Nude Mice
  • A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro
  • Oxygen Assessment in Tumors In Vivo using Phosphorescence Lifetime Imaging Microscopy
  • Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo
  • Measuring Pericellular Oxygen Tension for In Vitro Cell Culture
  • A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography
  • Photoacoustic Lifetime Imaging of Hypoxia
  • Staining Hypoxic Areas in Frozen and FFPE Tissue Sections with Hypoxyprobe™
  • Multiplex Immunofluorescence Staining Protocol for Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples
  • Detecting Hypoxia-Inducible Factor Levels and Activity
  • Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells
  • Measurement of Metabolic Alteration in Immune Cells under Hypoxia
  • Evaluation of Oxygen Consumption Rates In Situ
  • Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2-D and 3-D Tumor Models
  • Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells.