Proteases and Cancer Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Summary: | XII, 316 p. 80 illus., 60 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2024.
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| Edition: | 1st ed. 2024. |
| Series: | Methods in Molecular Biology,
2747 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-3589-6 |
| Format: | Electronic Book |
Table of Contents:
- Web-based resources to investigate protease function
- N-terminomics/TAILS of human cancer biopsies and cancer cell lines
- A new affinity-based probe to profile MMP active forms
- N-glycan insertion for probing protein-protein interactions and epitope mapping
- Expression and purification of recombinant ADAMTS8
- Expression and purification of active monomeric MMP7
- Mapping the binding sites of MMPs on type II and III collagens using triple-helical peptide Toolkits
- Determination of Versikine Levels by Enzyme-Linked Immunosorbent Assay (ELISA)
- Methods to investigate thrombin cleavage of osteopontin (OPN)
- Using siRNA silencing to analyze ADAM17 in macrophages
- Preparation and characterization of collagen-hyaluronic acid (Col-HA) matrices: in vitro mimics of the tumor microenvironment
- Assessment of Invadopodium Formation and Gelatin Degradation In Vitro
- Investigation of MT1-MMP activity in cancer cells
- Gelatinin situ zymography to study gelatinase activity in colon cancer cells treated with platelet microparticles (PMPs)
- Interrogating the impact of protease activity on tumor progression using 3D spheroid models
- Analysis of Matrix Metalloproteinase Activity and Inhibition in Cancer Spheroids
- Intravital microscopy to study the effect of matrix metalloproteinases inhibition on Acute myeloid leukemia cells migration in the bone marrow
- Fluorescence-based peptidolytic assay for high throughput screening of MMP14 inhibitors
- Generation of protease inhibitory antibodies by functional in vivo selection
- Engineering selective TIMPs using a counter-selective screening strategy
- Engineering new protease inhibitors using α2-macroglobulin
- Design of bioengineered peptides/proteases as anti-cancer reagents with integrated omics and machine-learning approaches. .