Bacterial Regulatory RNA Methods and Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Summary: | XII, 418 p. 97 illus., 77 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2024.
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| Edition: | 2nd ed. 2024. |
| Series: | Methods in Molecular Biology,
2741 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-3565-0 |
| Format: | Electronic Book |
Table of Contents:
- RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method
- Extraction and purification of outer membrane vesicles and their associated RNAs
- Analysis of phage regulatory RNAs: sequencing library construction from the fraction of small prokaryotic RNAs less than 50 nucleotides in length
- Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic
- Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus
- CRISPR interference-based functional small RNA genomics
- Investigation of sRNA-mRNA interactions in Bacillus subtilis in vivo
- In vitro methods for the investigation of sRNA-mRNA interactions in Bacillus subtilis
- RNA double helix hybridization measured by fluorescence correlation spectroscopy
- New perspectives on cross talks between bacterial regulatory RNAs from outer membrane vesicles and eukaryotic cells
- Experimental validation of RNA-RNA interactions by Electrophoretic Mobility Shift Assay
- Dynamics and function of sRNA/mRNAs under the scrutiny of computational simulation methods.-Analysis of sRNAs and their mRNA targets in Sinorhizobium meliloti: focus on half-live determinationEvaluation of 5'-end phosphorylation for small RNA stability and target regulation in vivo
- In-gel cyanoethylation for pseudouridines mass spectrometry detection of bacterial regulatory RNA
- Directed screening for sRNA targets in E. coli using a plasmid library
- Defining Bacterial RNA-RNA Interactomes Using CLASH
- Global identification of RNA-binding proteins in bacteria
- An integrated affinity chromatography-based approach to unravel the sRNA interactome in nitrogen-fixing rhizobia
- sRNA structural modeling based on NMR data
- Circular and linear dichroism for the analysis of small noncoding RNA properties.