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|a 9781071634011
|9 978-1-0716-3401-1
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|a 10.1007/978-1-0716-3401-1
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|a Zebrafish
|h [electronic resource] :
|b Methods and Protocols /
|c edited by James F. Amatruda, Corinne Houart, Koichi Kawakami, Kenneth D. Poss.
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|a 3rd ed. 2024.
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|a New York, NY :
|b Springer US :
|b Imprint: Humana,
|c 2024.
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|a XIV, 360 p. 94 illus., 85 illus. in color.
|b online resource.
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|a text
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|a online resource
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|b Table of contents navigation
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|b Single logical reading order
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|b Short alternative textual descriptions
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2707
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|a Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.
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|a This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
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|a Accessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
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|a No reading system accessibility options actively disabled
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|a Publisher contact for further accessibility information: accessibilitysupport@springernature.com
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|a Biological models.
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|a Biology
|x Technique.
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|a Biological Models.
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|a Biological Techniques.
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| 700 |
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|a Amatruda, James F.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 700 |
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|a Houart, Corinne.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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|a Kawakami, Koichi.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 700 |
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|a Poss, Kenneth D.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 710 |
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|a SpringerLink (Online service)
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|t Springer Nature eBook
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|i Printed edition:
|z 9781071634004
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| 776 |
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|i Printed edition:
|z 9781071634028
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|i Printed edition:
|z 9781071634035
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| 830 |
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2707
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-0716-3401-1
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| 912 |
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|a ZDB-2-PRO
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| 950 |
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|a Springer Protocols (Springer-12345)
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