Phage Display Methods and Protocols /

書目詳細資料
企業作者: SpringerLink (Online service)
其他作者: Hust, Michael (Editor), Lim, Theam Soon (Editor)
總結:XV, 605 p. 83 illus., 69 illus. in color.
text
語言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2023.
版:2nd ed. 2023.
叢編:Methods in Molecular Biology, 2702
主題:
在線閱讀:https://doi.org/10.1007/978-1-0716-3381-6
格式: 電子 電子書
書本目錄:
  • Antibody phage display
  • Construction of human immune and naive scFv Phage Display libraries
  • Construction of Naïve and Immune Human Fab Phage Display Library
  • Construction of synthetic antibody phage display libraries
  • Construction of chicken and ostrich antibody libraries
  • Construction of Rabbit Immune Antibody Libraries
  • Isolation and characterization of single-domain antibodies from immune phage display libraries
  • Phagekines: directed evolution and characterization of functional cytokines displayed on phages
  • Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly
  • Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification
  • Construction of semi-synthetic shark vNAR yeast surface display antibody libraries
  • Antibody selection via Phage Display in microtiter plates
  • Antibody selection in solution using magnetic beads
  • Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody Phage Display Biopanning
  • Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection
  • Antibody selection on cells targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach
  • Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS
  • Selection of affibody affinity proteins from phagemid libraries
  • Antibody affinity and stability maturation by error-prone PCR
  • Antibody batch cloning
  • Deep Mining of Complex Antibody Phage Pools
  • High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology
  • Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols
  • Mapping polyclonal antibody responses to infection using next generation phage display
  • Applications of high-throughput DNA sequencing to single-domain antibodydiscovery and engineering
  • Biomarker discovery by ORFeome Phage Display
  • Mapping Epitopes by Phage Display
  • Epivolve: A Protocol for Site-directed Antibodies.