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|a 9781071632994
|9 978-1-0716-3299-4
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|a 10.1007/978-1-0716-3299-4
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|a Flower Development
|h [electronic resource] :
|b Methods and Protocols /
|c edited by José Luis Riechmann, Cristina Ferrándiz.
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| 250 |
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|a 2nd ed. 2023.
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|a New York, NY :
|b Springer US :
|b Imprint: Humana,
|c 2023.
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| 300 |
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|a XV, 588 p. 86 illus., 67 illus. in color.
|b online resource.
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|a text
|b txt
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|a computer
|b c
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2686
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| 505 |
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|a Flower development in Arabidopsis -- Flower development in the Solanaceae -- The ABC of flower development in monocots: the model of rice spikelet -- Model species to investigate the origin of flowers -- Hormones and flower development in Arabidopsis -- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors -- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development -- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice -- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize -- Genetic and phenotypic analyses of carpel development in Arabidopsis -- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis -- Floral induction systems for the study of Arabidopsis flower development -- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types -- Protoplast Isolation for Plant Single-Cell RNA-seq -- Plant Nuclei Isolation for Single-nucleus RNA Sequencing -- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis -- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution -- The GUS reporter system in flower development studies -- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species -- Gene expression analysis by quantitative real-time PCR for floral tissues -- Misexpression approaches for the manipulation of flower development -- Genomic approaches for the study of flower development in floriculture crops -- Multi-omics methods applied to flower development -- Peptidomics methods applied to the study of flower development -- Quantifying gene expression domains in plant shoot apical meristems -- A NanoLuc-based transactivation assay in plants -- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.
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| 520 |
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|a This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
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| 650 |
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|a Botany.
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| 650 |
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4 |
|a Plant Science.
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| 700 |
1 |
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|a Riechmann, José Luis.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 700 |
1 |
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|a Ferrándiz, Cristina.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer Nature eBook
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| 776 |
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|i Printed edition:
|z 9781071632987
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| 776 |
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|i Printed edition:
|z 9781071633007
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| 776 |
0 |
8 |
|i Printed edition:
|z 9781071633014
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| 830 |
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2686
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-0716-3299-4
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| 912 |
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|a ZDB-2-PRO
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| 950 |
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|a Springer Protocols (Springer-12345)
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