Metabolic Reprogramming Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Papa, Salvatore (Editor), Bubici, Concetta (Editor)
Summary:XIV, 330 p. 1 illus.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2023.
Edition:1st ed. 2023.
Series:Methods in Molecular Biology, 2675
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-3247-5
Format: Electronic Book
Table of Contents:
  • In Vivo Tissue Lipid Uptake in Antisense Oligonucleotides (ASO) Treated Mice
  • In Vivo Hepatic Triglyceride Secretion Rate in Antisense Oligonucleotides (ASO) Treated Mice
  • Measurement of Fatty Acid Oxidation by High Resolution Respirometry: Special Considerations for Analysis of Skeletal and Cardiac Muscle and Adipose Tissue
  • In Vivo Imaging of Bone Marrow Long Chain Fatty Acid Uptake
  • Quantification and Tracing of Stable Isotope into Cysteine and Glutathione
  • Targeted Quantification of Amino Acids by Dansylation
  • Isolation of Mitochondria from Mouse Tissues for Functional Analysis.-Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liver Using MitoTimer Reporter Assay
  • Quantification of Intracellular ATP Content in Ex-Vivo GC B Cells.-UHPLC-HRMS-based Analysis of S-Hydroxymethyl-Glutathione, GSH and GSSG in Human Cells
  • Quantitation of glutathione and oxidized glutathione ratios from biological matrices using LC-MS/MS
  • Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells
  • Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue
  • 13C isotope labeling and mass spectrometric isotope enrichment analysis in acute brain slices
  • Metabolite analyses using Nuclear Magnetic Resonance (NMR) spectroscopy in plasma of patients with prostate cancer
  • Screening Kinase-Dependent Phosphorylation of Key Metabolic Reprogramming Regulators
  • Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPDH)
  • Quantitation of Glutamine Synthetase-1 Activity in Drosophila Melanogaster
  • Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation
  • Metabolic Reprogramming During B Cell Differentiation
  • An Integrated Methodology to Quantify the Glycolytic Stress in Plasma Cell Myeloma in Response to Cytotoxic Drugs
  • Spatial Analysis of Nucleotide Metabolism: From CRISPR Knock Out Cancer Cells to MALDI Imaging of Tumors
  • Assessment of Metabolic Pathways and Parameters in Extracellular Matrix-Detached Cells
  • Metabolic Networks: Weighted Gene Correlation Network Analysis.