Transposable Elements Methods and Protocols /

Dades bibliogràfiques
Autor corporatiu: SpringerLink (Online service)
Altres autors: Branco, Miguel R. (Editor), de Mendoza Soler, Alexandre (Editor)
Sumari:XII, 382 p. 65 illus., 60 illus. in color.
text
Idioma:anglès
Publicat: New York, NY : Springer US : Imprint: Humana, 2023.
Edició:1st ed. 2023.
Col·lecció:Methods in Molecular Biology, 2607
Matèries:
Accés en línia:https://doi.org/10.1007/978-1-0716-2883-6
Format: Electrònic Llibre
Taula de continguts:
  • An overview of best practices for transposable element identification, classification and annotation in eukaryotic genomes
  • Assembly-free annotation and quantification of transposable elements with dnaPipeTE
  • Best practice for the identification of horizontally transferred transposons
  • Genotyping of transposable elements insertions segregating in the human populations using short-read re-alignments
  • A Pangenome approach to detect and genotype TE insertion polymorphisms.0 Experimental validation of transposable element insertions using the Polymerase Chain Reaction (PCR)
  • Quantification of LINE-1 RNA expression from bulk RNA-seq using L1EM
  • Genome-wide profiling of L1 DNA methylation by bs-ATLAS-seq
  • Nanopore epigenomic analysis of transposable element DNA modifications
  • Targeted resequencing and methylation analysis of L1 elements by nanopore sequencing
  • Inferring protein-DNA binding profiles at interspersed repeats using HiChIP and PatChER
  • Affinity-based Interactome Analysis of Endogenous LINE-1 Macromolecules
  • LINE-1 retrotransposition assays in embryonic stem cells
  • Detecting somatic transposable element insertions in Drosophila tissues
  • Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering
  • Epigenetic editing of transposable and repetitive elements
  • Using CRISPR to investigate the regulatory activity of transposable elements. .