Fluorescent Proteins Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Sharma, Mayank (Editor)
Summary:XI, 326 p. 95 illus., 80 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2023.
Edition:1st ed. 2023.
Series:Methods in Molecular Biology, 2564
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-2667-2
Format: Electronic Book
Table of Contents:
  • Using FPbase: The Fluorescent Protein Database
  • Selecting the Fluorescent Protein for In Vivo Imaging Experiments
  • Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo
  • Directed Evolution of Fluorescent Proteins in Bacteria
  • Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation
  • Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization
  • Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
  • CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line
  • Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants
  • Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
  • DNA Visualization Using Fluorescent Proteins
  • Quantification ofIntracellular Citrate Concentrations with Genetically Encoded Biosensors
  • Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out
  • Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae
  • Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells
  • Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins
  • Determining Protein-Protein Interaction with GFP-Trap Beads.