Archaea Methods and Protocols /
| 団体著者: | |
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| その他の著者: | |
| 要約: | XIV, 549 p. 98 illus., 88 illus. in color. text |
| 言語: | 英語 |
| 出版事項: |
New York, NY :
Springer US : Imprint: Humana,
2022.
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| 版: | 1st ed. 2022. |
| シリーズ: | Methods in Molecular Biology,
2522 |
| 主題: | |
| オンライン･アクセス: | https://doi.org/10.1007/978-1-0716-2445-6 |
| フォーマット: | 電子媒体 図書 |
目次:
- Archaea: a goldmine for molecular biologists and evolutionists
- Progress and challenges in archaeal genetic manipulation
- Genetic manipulation of Haloferax species
- CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii
- Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis
- Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea
- An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ΔH
- Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius
- A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus
- Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus
- Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales
- Progress and challenges in archaeal molecular biology
- ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery
- Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii
- Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii
- Quantitative Mass Spectrometry by SILAC in Haloferax volcanii
- Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach
- Proteomic sample preparation and data analysis in line with the Archaeal Proteome
- In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii
- Proteolytic activity assays in haloarchaea
- Carotenoids from Haloarchaea: Extraction, fractionation and characterization
- Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii
- Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax
- Progress and Challenges in Archaeal Cellular Biology Analysis
- Methods to Analyze Motility in Eury- And Crenarchaea
- Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii
- Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions
- Cell Adhesion and Biofilm Formation Analysis
- BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging
- Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea
- Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation
- Progress and Challenges in Studying the Ecophysiology of Archaea
- Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes
- Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii.