Yeast Metabolic Engineering Methods and Protocols /
| Korporativní autor: | |
|---|---|
| Další autoři: | , |
| Shrnutí: | XI, 295 p. 51 illus., 32 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
New York, NY :
Springer US : Imprint: Humana,
2022.
|
| Vydání: | 2nd ed. 2022. |
| Edice: | Methods in Molecular Biology,
2513 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-0716-2399-2 |
| Médium: | Elektronický zdroj Kniha |
Obsah:
- An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae
- High Efficiency Plasmid DNA Transformation in Yeast
- CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae
- Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening
- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
- Competent Cell Preparation and Transformation of Pichia pastoris
- CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
- Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
- Engineering of Promoters for Gene Expression in Pichia pastoris
- Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory
- Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica
- A Universal Gene Expression System for Novel Yeast Species
- Dynamic Feeding of Pichia pastoris
- Automated Evolutionary Engineering of Yeasts
- Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.