Yeast Metabolic Engineering Methods and Protocols /

书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Mapelli, Valeria (Editor), Bettiga, Maurizio (Editor)
总结:XI, 295 p. 51 illus., 32 illus. in color.
text
语言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2022.
版:2nd ed. 2022.
丛编:Methods in Molecular Biology, 2513
主题:
在线阅读:https://doi.org/10.1007/978-1-0716-2399-2
格式: 电子 图书
书本目录:
  • An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae
  • High Efficiency Plasmid DNA Transformation in Yeast
  • CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae
  • Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening
  • Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
  • Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
  • Competent Cell Preparation and Transformation of Pichia pastoris
  • CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
  • Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
  • Engineering of Promoters for Gene Expression in Pichia pastoris
  • Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory
  • Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica
  • A Universal Gene Expression System for Novel Yeast Species
  • Dynamic Feeding of Pichia pastoris
  • Automated Evolutionary Engineering of Yeasts
  • Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.