The P2X7 Receptor Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Nicke, Annette (Editor)
Summary:XIV, 394 p. 93 illus., 81 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2022.
Edition:1st ed. 2022.
Series:Methods in Molecular Biology, 2510
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-2384-8
Format: Electronic Book
Table of Contents:
  • How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating
  • Agonists, Antagonists, and Modulators of P2X7 Receptors
  • Modulation of Cell Energy Metabolism by the P2X7 Receptor
  • Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE
  • Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4)
  • Development of Antibody and Nanobody Tools for P2X7
  • Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors
  • Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations
  • Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes
  • A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors
  • Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents
  • Using SymmetricalOrganic Cation Solutions to Study P2X7 Ion Permeation
  • Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors
  • Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry
  • Characterization of P2X7 Receptors in Human Blood Cells
  • Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube
  • Administration of P2X7 Receptor Blockers in Oncological Experimental Models
  • Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease
  • Analysis of P2X7-Induced Neuronal Branching
  • Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique
  • Analyzing the Role of the P2X7 Receptor in Epilepsy.