piRNA Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Parrish, Nicholas F. (المحرر), Iwasaki, Yuka W. (المحرر)
الملخص:XII, 397 p. 83 illus., 73 illus. in color.
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اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2022.
الطبعة:1st ed. 2022.
سلاسل:Methods in Molecular Biology, 2509
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-0716-2380-0
التنسيق: الكتروني كتاب الكتروني
جدول المحتويات:
  • Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides
  • CRISPR-Mediated Genome Engineering in Aedes aegypti
  • PIWI-Directed DNA Elimination for Tetrahymena Genetics
  • Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing
  • Isolation and Processing of Bovine Oocytes for Small RNA Sequencing
  • 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther
  • Cloning, Sequencing, and Linkage Analysis of piRNAs
  • Drosophila Genetic Resources for Elucidating piRNA Pathway
  • Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System
  • Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline
  • CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells
  • Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability
  • Revisiting the Glass Treatmentfor Single Molecule Analysis of ncRNA Function
  • Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification
  • Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq
  • UPA-Seq-Based Search Method for Functional lncRNA Candidates
  • Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST
  • Computational Methods for the Discovery and Annotation of Viral Integrations
  • Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-Coding RNAs
  • Extending and Running the Mosquito Small RNA Genomics Resource Pipeline
  • Preparation of Non-Overlapping Transposable Elements (TE) Annotation by Interval Tree
  • Statistical Thermodynamics Approach for Intracellular Phase Separation.