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|a 9781071623800
|9 978-1-0716-2380-0
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|a 10.1007/978-1-0716-2380-0
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|a piRNA
|h [electronic resource] :
|b Methods and Protocols /
|c edited by Nicholas F. Parrish, Yuka W. Iwasaki.
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| 250 |
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|a 1st ed. 2022.
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| 264 |
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|a New York, NY :
|b Springer US :
|b Imprint: Humana,
|c 2022.
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| 300 |
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|a XII, 397 p. 83 illus., 73 illus. in color.
|b online resource.
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|a text
|b txt
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|a computer
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|a text file
|b PDF
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2509
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| 505 |
0 |
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|a Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides -- CRISPR-Mediated Genome Engineering in Aedes aegypti -- PIWI-Directed DNA Elimination for Tetrahymena Genetics -- Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing -- Isolation and Processing of Bovine Oocytes for Small RNA Sequencing -- 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther -- Cloning, Sequencing, and Linkage Analysis of piRNAs -- Drosophila Genetic Resources for Elucidating piRNA Pathway -- Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System -- Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline -- CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells -- Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability -- Revisiting the Glass Treatmentfor Single Molecule Analysis of ncRNA Function -- Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification -- Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq -- UPA-Seq-Based Search Method for Functional lncRNA Candidates -- Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST -- Computational Methods for the Discovery and Annotation of Viral Integrations -- Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-Coding RNAs -- Extending and Running the Mosquito Small RNA Genomics Resource Pipeline -- Preparation of Non-Overlapping Transposable Elements (TE) Annotation by Interval Tree -- Statistical Thermodynamics Approach for Intracellular Phase Separation.
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| 520 |
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|a This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.
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| 650 |
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|a Biomaterials.
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| 650 |
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|a Nucleic acids.
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| 650 |
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|a Proteins .
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|a Nucleic Acid.
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| 650 |
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|a Protein Biochemistry.
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| 700 |
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|a Parrish, Nicholas F.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 700 |
1 |
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|a Iwasaki, Yuka W.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer Nature eBook
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| 776 |
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|i Printed edition:
|z 9781071623794
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| 776 |
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|i Printed edition:
|z 9781071623817
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| 776 |
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|i Printed edition:
|z 9781071623824
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| 830 |
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 2509
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-0716-2380-0
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| 912 |
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|a ZDB-2-PRO
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| 950 |
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|a Springer Protocols (Springer-12345)
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