African Swine Fever Virus Methods and Protocols /
| Müşterek Yazar: | |
|---|---|
| Diğer Yazarlar: | |
| Özet: | XII, 218 p. 44 illus., 41 illus. in color. text |
| Dil: | İngilizce |
| Baskı/Yayın Bilgisi: |
New York, NY :
Springer US : Imprint: Humana,
2022.
|
| Edisyon: | 1st ed. 2022. |
| Seri Bilgileri: | Methods in Molecular Biology,
2503 |
| Konular: | |
| Online Erişim: | https://doi.org/10.1007/978-1-0716-2333-6 |
| Materyal Türü: | Elektronik Kitap |
İçindekiler:
- Working safely with African swine fever virus
- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus
- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope
- Primary macrophage culture from porcine blood and lungs
- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses
- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction
- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks
- Genotyping of African swine fever virus
- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag)
- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS)
- African Swine Fever Virus Haemadosrption Inhibition Assay
- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells
- Purification of African swine fever virus
- African swine fever virus plaque assay and disinfectant testing
- Bioorthogonal labelling of African swine fever virus infected cells
- Whole genome sequencing of African swine fever virus.