TGF-Beta Signaling Methods and Protocols /

书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Zi, Zhike (Editor), Liu, Xuedong (Editor)
总结:X, 239 p. 49 illus., 45 illus. in color.
text
语言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2022.
版:1st ed. 2022.
丛编:Methods in Molecular Biology, 2488
主题:
在线阅读:https://doi.org/10.1007/978-1-0716-2277-3
格式: 电子 图书
书本目录:
  • Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot
  • Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter
  • Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP
  • Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity
  • Visualizing Dynamic Changes during TGF-β-Induced Epithelial to Mesenchymal Transition
  • Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-β Family Signaling in Human Breast Cancer Progression
  • Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling
  • CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators
  • Optogenetic Control of TGF-β Signaling
  • Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells
  • Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network
  • Discrete Logic Modeling of Cell Signaling Pathways
  • Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering
  • Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst
  • Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs.