Cell Polarity Signaling Methods and Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Summary: | XIV, 530 p. 124 illus., 105 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2022.
|
| Edition: | 1st ed. 2022. |
| Series: | Methods in Molecular Biology,
2438 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-2035-9 |
| Format: | Electronic Book |
Table of Contents:
- Use of Fluorescence Recovery after Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins
- FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
- Live‐Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics
- Single-Cell Single Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates
- Biochemical Assays to Detect Activation of Small GTPases Rho, Rac, and Cdc42 during Morphogenesis
- Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos
- Regulation of Cell Polarity by Posttranslational Protein Palmitoylation
- Enrichment and Detection of Wnt Proteins from Cell Culture Media
- Using Live-Imaging to Examine Early Cardiac Development in Zebrafish
- Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm
- In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis
- Avian Embryosas a Model to Study Vascular Development
- BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice
- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics
- Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo
- Methods to Investigate Cell Polarity of Inner Ear
- Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice
- In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance
- Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance
- Dissection, Fixation, and Immunostaining of the Drosophila Midgut
- In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands
- Imaging Epidermal Cell Rearrangement in the C. elegans Embryo
- Methods for the Study of Apical Constriction during Ascidian Gastrulation
- Assays for Apical Constriction Using the Xenopus Model
- The Use of 3-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation
- Studying Cell Polarity Dynamics during Cancer Initiation Using Inducible 3D Organotypic Cultures
- Under-Agarose Chemotaxis and Migration Assays for Dictyostelium
- Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis
- Targeted Injections, Atomic Force Microscopy, and Hydrogels: A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo
- Quantitative Analysis of Directional Neural Crest Cell Migration.