Cell-Free Gene Expression Methods and Protocols /

Podrobná bibliografie
Korporativní autor: SpringerLink (Online service)
Další autoři: Karim, Ashty S. (Editor), Jewett, Michael C. (Editor)
Shrnutí:XV, 435 p. 80 illus., 63 illus. in color.
text
Jazyk:angličtina
Vydáno: New York, NY : Springer US : Imprint: Humana, 2022.
Vydání:1st ed. 2022.
Edice:Methods in Molecular Biology, 2433
Témata:
On-line přístup:https://doi.org/10.1007/978-1-0716-1998-8
Médium: Elektronický zdroj Kniha
Obsah:
  • Best Practices for DNA Template Preparation towards Improved Reproducibility in Cell-Free Protein Production
  • Simple Extract Preparation Methods for E. coli-Based Cell-Free Expression
  • Preparation and Screening of Cell-Free Extract from Non-Growing Escherichia coli A19 Cells
  • Cell-Free Protein Synthesis Using Pichia pastoris
  • A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression
  • In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins
  • High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems
  • Cell-Free Gene Expression from DNA Brushes
  • Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications
  • Measurement of Transcription, Translation, and Other Enzymatic Processes during Cell-Free Expression Using PERSIA
  • Cell-Free Noncanonical Redox Cofactor Systems
  • Cell-Free Protein Synthesis forHigh-Throughput Biosynthetic Pathway Prototyping
  • Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry
  • Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus
  • Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression
  • Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles
  • Cell-Free Synthesis Strategies to Probe Co-Translational Folding of Proteins within Lipid Membranes
  • Assembly of RNA Nanostructures from Double Crossover Tiles
  • Cell-Free Biosensors and AI Integration
  • ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors
  • Optical Sensing in Cell-Free Expression
  • Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers
  • Detection of Norovirus Using Paper-Based Cell-Free Systems
  • A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes
  • Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology.