Atherosclerosis Methods and Protocols /
| מחבר תאגידי: | |
|---|---|
| מחברים אחרים: | |
| סיכום: | XXII, 880 p. 154 illus., 136 illus. in color. text |
| שפה: | אנגלית |
| יצא לאור: |
New York, NY :
Springer US : Imprint: Humana,
2022.
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| מהדורה: | 1st ed. 2022. |
| סדרה: | Methods in Molecular Biology,
2419 |
| נושאים: | |
| גישה מקוונת: | https://doi.org/10.1007/978-1-0716-1924-7 |
| פורמט: | אלקטרוני ספר אלקטרוני |
תוכן הענינים:
- Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions
- Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation
- Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis
- Survey of approaches for investigation of atherosclerosis in vivo
- Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis
- Peptides as therapeutic agents for atherosclerosis
- Isolation and culturing of primary mouse and human macrophages
- Effective transfection and gene silencing of primary murine macrophages with small interfering RNA
- Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques invitro
- Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis
- Chick embryo ex vivo aortic sprouting assays for cardiovascular research
- Purification and analysis of circulating lipid particles
- Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research
- Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation
- A fluorescent based in vitro method to assess cholesterol efflux
- Monitoring the anisotropy and fluidity of the HDL monolayer as surrogates of HDL functionality
- Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis
- Dual-fluorescence in vitro efferocytosis assay
- Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis
- Probing inflammasome activation in atherosclerosis
- Investigation of platelet aggregation in atherosclerosis
- Extracellular vesicles in atherosclerosis research
- The use of wire myography to investigate vascular tone and function
- Pig and mouse models of hyperlipidemia and atherosclerosis
- Use of rabbit models to study atherosclerosis
- Genetically engineered hamster models of dyslipidemia and atherosclerosis
- Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9
- Monitoring leukocyte migration during atherosclerosis in vivo
- Evaluation of plaque burden and lipid content in atherosclerotic plaques
- Monitoring cellularity and expression of key markers in atherosclerotic plaques
- Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings
- Investigation of atherosclerotic plaque vulnerability
- Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis
- Assessing efferocytosis in atherosclerotic lesions in situ
- Monitoring atheroprotective macrophage cholesterol efflux in vivo
- Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis
- Whole mount preparation of mouse aorta for confocal microscopy studies of the intima
- Isolation of mouse aortic RNA for transcriptomics
- Redox proteomics analysis of atherosclerotic aortas: Application of the “OxICAT” method
- Intravital microscopy in atherosclerosis research
- Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization
- In vivo gene editing in lipid and atherosclerosis research
- Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research
- Combined single cell RNA and single cell α/β T cell receptor sequencing of the arterial wall in atherosclerosis
- Tissue clearing approaches in atherosclerosis
- Single cell antibody sequencing in atherosclerosis research
- Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries
- Contrast-enhanced ultrasound molecular imaging in atherosclerosis
- Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research
- Positron emission tomography in atherosclerosis research
- Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy
- Intravascular fluorescence molecular imaging of atherosclerosis. .