Single-Cell Protein Analysis Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ooi, Aik T. (Editor)
Summary:X, 311 p. 95 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2022.
Edition:1st ed. 2022.
Series:Methods in Molecular Biology, 2386
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-1771-7
Format: Electronic Book
Table of Contents:
  • Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections
  • Indirect Immunofluorescence of Tissue Sections
  • Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique
  • High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry
  • Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot)
  • Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot
  • Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing
  • Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis
  • Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels
  • High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging
  • Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA
  • High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform
  • Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform
  • Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets
  • Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits
  • Combined Measurement of RNA and Protein Expression on a Single-Cell Level
  • Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.