The Unfolded Protein Response Methods and Protocols /
| Korporativní autor: | |
|---|---|
| Další autoři: | |
| Shrnutí: | XII, 332 p. 64 illus., 53 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
New York, NY :
Springer US : Imprint: Humana,
2022.
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| Vydání: | 1st ed. 2022. |
| Edice: | Methods in Molecular Biology,
2378 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-0716-1732-8 |
| Médium: | Elektronický zdroj Kniha |
Obsah:
- Measuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues
- Fluorescence-based biosensors for the detection of the unfolded protein response
- Indirect methods to measure unfolded proteins in living cells using fluorescent proteins
- A platform technology for monitoring the Unfolded Protein Response
- The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts
- High-throughput analysis of protein turnover with tandem fluorescent protein timers
- Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae
- Determination of protein translation during the activation of the UPR
- Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR
- Assays to study IRE1 activation and signaling
- Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis
- Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast
- Analyze mouse knockout models of UPR pathway elements
- In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS
- Detection of PERK signaling in the central nervous system
- Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans
- Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo
- Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana
- Structure-based drug discovery of IRE1 modulators
- Chemical screening to detect ER stress regulators.