Next Generation Culture Platforms for Reliable In Vitro Models Methods and Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , , |
| Summary: | XII, 307 p. 69 illus., 59 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2021.
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| Edition: | 1st ed. 2021. |
| Series: | Methods in Molecular Biology,
2273 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-1246-0 |
| Format: | Electronic Book |
Table of Contents:
- Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
- Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids
- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation
- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors
- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres
- Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models
- Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues
- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models
- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold
- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells
- 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids
- Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics
- Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC)
- Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns
- Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer
- Isolation, Characterization, and MicroRNAs Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids
- Biomimetic 3D-Bone Tissue Model
- Using the Air-Liquid Interface Approach to Foster Apical-Basal Polarization of Mammalian FemaleReproductive Tract Epithelia In Vitro
- Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa
- Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro.