Cell Cycle Checkpoints Methods and Protocols /
| Korporativní autor: | |
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| Další autoři: | |
| Shrnutí: | XI, 242 p. 31 illus., 21 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
New York, NY :
Springer US : Imprint: Humana,
2021.
|
| Vydání: | 1st ed. 2021. |
| Edice: | Methods in Molecular Biology,
2267 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-0716-1217-0 |
| Médium: | Elektronický zdroj E-kniha |
Obsah:
- Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy
- Detection of Post-Translationally Modified p53 by Western Blotting
- Global Analyses to Identify Direct Transcriptional Targets of p53
- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay
- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors
- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids
- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer
- The DNA Damage Response in Xenopus laevis Cell-Free Extracts
- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation
- Knockdown of Target Genes by siRNA In Vitro
- Assaying Cell Cycle Status Using Flow Cytometry
- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues
- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast
- Calreticulin Exposure in Mitotic Catastrophe
- Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy
- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.