Cell Cycle Checkpoints Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Manfredi, James J. (المحرر)
الملخص:XI, 242 p. 31 illus., 21 illus. in color.
text
اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2021.
الطبعة:1st ed. 2021.
سلاسل:Methods in Molecular Biology, 2267
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-0716-1217-0
التنسيق: الكتروني كتاب الكتروني
جدول المحتويات:
  • Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy
  • Detection of Post-Translationally Modified p53 by Western Blotting
  • Global Analyses to Identify Direct Transcriptional Targets of p53
  • Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay
  • Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors
  • DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids
  • A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer
  • The DNA Damage Response in Xenopus laevis Cell-Free Extracts
  • Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation
  • Knockdown of Target Genes by siRNA In Vitro
  • Assaying Cell Cycle Status Using Flow Cytometry
  • Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues
  • Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast
  • Calreticulin Exposure in Mitotic Catastrophe
  • Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy
  • Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.