Melanoma Methods and Protocols /

מידע ביבליוגרפי
מחבר תאגידי: SpringerLink (Online service)
מחברים אחרים: Hargadon, Kristian M. (Editor)
סיכום:XX, 674 p. 122 illus., 97 illus. in color.
text
שפה:אנגלית
יצא לאור: New York, NY : Springer US : Imprint: Humana, 2021.
מהדורה:1st ed. 2021.
סדרה:Methods in Molecular Biology, 2265
נושאים:
גישה מקוונת:https://doi.org/10.1007/978-1-0716-1205-7
פורמט: אלקטרוני ספר אלקטרוני
תוכן הענינים:
  • Model Systems for the Study of Malignant Melanoma
  • Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing
  • A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix
  • Wound Healing Assay for Melanoma Cell Migration
  • A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells
  • Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology
  • Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry
  • Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry
  • In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium
  • A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells
  • An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence
  • Evaluating Melanoma Viability and Proliferation in 3D Microenvironments
  • Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models
  • Enrichment of Melanoma Cancer Stem Cells via Sphere Assays
  • Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry
  • Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells
  • PD-L1 Detection on Circulating Melanoma Cells
  • Transcript-Based Detection of Circulating Melanoma Cells
  • Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients
  • Simultaneous BRAFV600E Protein and DNA Aberration Detection in Circulating Melanoma Cells Using an Integrated Multi-Molecular Sensor
  • Single Cell Analysis of BRAFV600E and NRASQ61R Mutation Status in Melanoma Cell Lines as Method Generation for Circulating Melanoma Cells
  • A Rapid Exosome Isolation Using Size Exclusion Chromatography(REIUS) Method for Exosome Isolation from Melanoma Cell Lines
  • Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients
  • An Immunocapture-Based Assay for Detecting Multiple Antigens in Melanoma-Derived Extracellular Vesicles
  • Post-Lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients
  • Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins
  • Detection of Melanoma Cells in Lymphatic Drainage (LD) after Lymph Nodes Dissection via Nested RT-PCR Analysis of Molecular Melanocytic Markers
  • A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue
  • PET Imaging of Melanoma Using Melanin-Targeted Probe
  • Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis
  • Salivary Proteomic Analysis of Canine Oral Melanoma by MALDI-TOF Mass Spectrometry and LC-Mass Spectrometry/Mass Spectrometry
  • Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers
  • Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma
  • Assessment of Cell-Free microRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients’ Blood
  • High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0
  • Immunotyping and Quantification of Melanoma Tumor–Infiltrating Lymphocytes
  • Single Cell Gene Expression, Clonality, and Feature Barcoding of Melanoma Tumor Infiltrating Lymphocytes
  • Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma
  • Multiplex Immunohistochemistry Analysis of Melanoma Tumor Infiltrating Lymphocytes
  • Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures
  • The Use of Nanoparticles in the Delivery of Nucleic Acids for Melanoma Treatment
  • siRNA Delivery to Melanoma Cells with Cationic Niosomes
  • Controlled Delivery of Plasmid DNA to Melanoma Tumors by Gene Electrotransfer
  • Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma
  • Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy.