Proteomic Profiling Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Summary: | XV, 592 p. 131 illus., 91 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2021.
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| Edition: | 2nd ed. 2021. |
| Series: | Methods in Molecular Biology,
2261 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-1186-9 |
| Format: | Electronic Book |
Table of Contents:
- How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi
- Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis
- Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis
- Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry
- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation
- Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins
- Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain
- Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing
- A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
- Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma
- Isolation of Extracellular Vesicles for Proteomic Profiling
- Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses
- Flow Cytometry as an Important Tool in Proteomic Profiling
- Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets
- Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors
- Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks
- Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma
- Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development
- cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection
- Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions
- Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis
- Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method
- Studying OTUD6B-OTUB1 Protein-Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays
- Thermal Shift Assay for Exploring Interactions between Fatty Acid-Binding Protein and Inhibitors
- Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses
- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling
- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model
- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology
- Technical Considerations for Contemporary Western Blot Techniques
- Simple Western: Bringing the Western Blot into the 21st Century
- Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
- Sample Preparation of Secreted Mammalian Host Cell Proteins and their Characterization by Two-Dimensional Electrophoresis and Western Blotting
- Quantitative Proteomic Analysis Using Formalin-Fixed Paraffin-Embedded (FFPE) Human Cardiac Tissue
- Chloroplast Isolation and Enrichment of Low Abundant Proteins by Affinity Chromatography for Identification in Complex Proteomes
- Principles of Protein Labeling Techniques
- Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.