Skeletal Development and Repair Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Summary: | XV, 482 p. 125 illus., 105 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Humana,
2021.
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| Edition: | 2nd ed. 2021. |
| Series: | Methods in Molecular Biology,
2230 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-0716-1028-2 |
| Format: | Electronic Book |
Table of Contents:
- Overview of Skeletal Development
- Overview of Skeletal Repair (Fracture Healing and Its Assessment)
- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research
- Generation of Closed Transverse Fractures in Small Animals
- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes
- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse
- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton
- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development
- Murine Limb Explant Cultures to Assess Cartilage Development
- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair
- MicroCT for Scanning and Analysis of Mouse Bones
- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties
- Phenotyping Intact Mouse Bones using Bone CLARITY
- Processing and Sectioning Undecalcified Murine Bone Specimens
- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020)
- Demineralized Murine Skeletal Histology
- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix
- Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter
- Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue
- Detection of Hypoxic Regions in the Bone Microenvironment
- EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections
- Whole-mount In Situ Hybridization in Murine Tissues
- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo
- Isolation and Culture of Periosteum Derived Progenitor Cells from Mice
- Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage
- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts.-Mitochondrial Function and Metabolism of Cultured Skeletal Cells
- Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants
- RANKL-based Osteoclastogenic Assay from Murine Bone Marrow Cells
- Hematopoietic Stem Cell Cultures and Assays. .