RNA Tagging Methods and Protocols /

書目詳細資料
企業作者: SpringerLink (Online service)
其他作者: Heinlein, Manfred (Editor)
總結:XIV, 490 p. 91 illus., 77 illus. in color.
text
語言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2020.
版:1st ed. 2020.
叢編:Methods in Molecular Biology, 2166
主題:
在線閱讀:https://doi.org/10.1007/978-1-0716-0712-1
格式: 電子 電子書
書本目錄:
  • Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization
  • Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution
  • Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells
  • Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence
  • Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More
  • Visualization of Transiently Expressed mRNA in Plants Using MS2
  • New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells
  • In Vivo Imaging of Mobile mRNAs in Plants Using MS2
  • RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi
  • Utilizing Potato Virus X to Monitor RNA Movement
  • A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging,Cell Sorting, and Sequencing
  • Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake
  • Labeling of dsRNA for Fungal Uptake Detection Analysis
  • Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs)
  • Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes
  • A Single-Molecule RNA Mobility Assay to Identify Proteins that Link RNAs to Molecular Motors
  • Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale
  • Double-Stranded RNA Pull-Down to Characterize Viral Replication Complexes in Plants
  • CRISPR Guide RNA Design Guidelines for Efficient Genome Editing
  • Live Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA
  • Live-Cell Imaging of Genomic Loci Using CRISPR/Molecular Beacon Hybrid Systems
  • Using RNA Tags for Multicolor Live Imaging of Chromatin Loci andTranscription in Drosophila Embryos
  • Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs across Plant Species
  • Transfection of Small Non-Coding RNAs into Arabidopsis thaliana Protoplasts
  • In Vivo Reporter System for miRNA-Mediated RNA Silencing
  • TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Non-Coding RNAs
  • Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled RNA for Conformational Analysis and Imaging.