Basophils and Mast Cells Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Gibbs, Bernhard F. (Editor), Falcone, Franco H. (Editor)
Summary:XVIII, 380 p. 50 illus., 48 illus. in color.
text
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2020.
Edition:2nd ed. 2020.
Series:Methods in Molecular Biology, 2163
Subjects:
Online Access:https://doi.org/10.1007/978-1-0716-0696-4
Format: Electronic eBook
Table of Contents:
  • Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease
  • Purification of Basophils from Peripheral Human Blood
  • Mast Cell Purification Protocols
  • Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells
  • Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs)
  • Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34+ Progenitor Cells
  • Generation of Mast Cells from Murine Stem Cell Progenitors
  • Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model
  • The Absolute Basophil Count
  • Mast Cell and Basophils Cell Lines: A Compendiu
  • Use of Humanized RBL Reporter Systems for Detection of Allergen-specific IgE Sensitization in Human Serum
  • Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for Detection of Allergen-specific IgE in Patient Sera Using Allergen Microarrays
  • NPY-mRFP Rat Basophil Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation
  • Use of Engineered Nanoparticles (ENPs) for the Study of High-affinity IgE Receptor FcεRI Receptor Engagement and Rat Basophil Leukemia (RBL) Cell degranulation
  • Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques
  • Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid
  • Basophil Activation Techniques: Staining of Exteriorized Granule Matrix
  • Measurement and Functional Analysis of the Mas-related G protein-coupled receptor MRGPRX2 on Human Mast Cells and Basophils
  • The Mast Cell Activation Test (MAT)
  • Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique
  • Measuring Histamine and Cytokine Release from Basophils and Mast Cells
  • Detection of Mast Cells and Basophils by Immunohistochemistry
  • Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry
  • Mast Cell Migration and Chemotaxis Assayed by Microscopy
  • Basophil Stimulation and Signaling Pathway
  • Gene Silencing Approaches in Mast Cells and Primary Human Basophils
  • Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells
  • Mast Cell Mediated Reactions in vivo
  • Identification of Murine Basophils by Flow Cytometry and Histology. .