Cerebral Cavernous Malformations (CCM) Methods and Protocols /
| مؤلف مشترك: | |
|---|---|
| مؤلفون آخرون: | , , |
| الملخص: | XV, 496 p. 86 illus., 62 illus. in color. text |
| اللغة: | الإنجليزية |
| منشور في: |
New York, NY :
Springer US : Imprint: Humana,
2020.
|
| الطبعة: | 1st ed. 2020. |
| سلاسل: | Methods in Molecular Biology,
2152 |
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://doi.org/10.1007/978-1-0716-0640-7 |
| التنسيق: | الكتروني كتاب الكتروني |
جدول المحتويات:
- From Genes and Mechanisms to Molecular-targeted Therapies: the Long Climb to the Cure of Cerebral Cavernous Malformation (CCM) Disease
- Incidence, Prevalence, and Clinical Presentation of Cerebral Cavernous Malformations
- Natural History, Clinical, and Surgical Management of Cavernous Malformations
- Molecular Genetics Screening of CCM patients, An Overview
- Next Generation Sequecing (NGS) Strategies for Genetic Testing and Diagnosis of Cerebral Cavernous Malformation (CCM) Disease
- Genome-wide Genotyping of Cerebral Cavernous Malformation Type 1 Individuals to Identify Genetic Modifiers of Disease Severity
- Clinical Imaging of Cerebral Cavernous Malformations: Computed Tomography and Magnetic Resonance Imaging
- Neuroradiology: Differential Diagnosis, Follow-Up, and Reporting
- Surgical Management of Brain Cavernous Malformations
- Generation of CCM Phenotype by a Human Microvascular Endothelial Model
- Isolation and Purification of Mouse Brain Endothelial Cells to Study Cerebral Cavernous Malformation Disease
- Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease
- CRISPR/Cas9-mediated Generation of Human Endothelial Cell Knockout Models of CCM Disease
- Dissection of the Role of CCM Genes in Tubulogenesis using the Drosophila Tracheal System as a Model
- Generation and Analysis of CCM Phenotypes in C. elegans
- Generation of Transgenic Lines of Zebrafish Expressing Fluorescently-tagged CCM Proteins to Study Their Function and Subcellular Localization within the Vasculature
- Vertebrate Models to Investigate CCM Pathogenesis: The Zebrafish and Mouse Model
- Generation of Cerebral Cavernous Malformation in Neonatal Mouse Models using Inducible Cre-LoxP Strategy
- Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain
- Identification of the KRIT1 Protein by LexA-based Yeast Two-Hybrid System
- Crystallographic Studies of the Cerebral Cavernous Malformations Proteins
- Microscopy Techniques to Investigate CCM Pathogenesis
- Preparation and Analysis of Protein Extracts to Investigate CCM Pathogenesis
- Systems Wide Analysis of CCM Signaling Complex (CSC) Alterations in CCM Deficient Models using Omics Approaches
- Study of Molecular Interactions of CCM Proteins by using a GAL4-based Yeast Two-hybrid Screening
- Study of CCM Microvascular Endothelial Phenotype by an in vitro Tubule Differentiation Model
- Bidimentional in vitro Angiogenic Assays to Study CCM Pathogenesis: Endothelial Cell Proliferation and Migration
- Measurement of Endothelial Barrier Function in Mouse Models of Cerebral Cavernous Malformations using Intravital Microscopy
- Immunofluorescence of Cell-Cell and Cell-Extracellular Matrix Adhesive Defects in in vitro Endothelial CCM Model. Juxtacrine Role of Mutant Extracellular Matrix on Wild-Type Endothelial Cells
- Detection of p62/SQSTM1Aggregates in Cellular Models of CCM Disease by Immunofluorescence
- Notch Signaling in Familial Cerebral Cavernous Malformations and Immunohistochemical Detection of cleaved Notch1 Intracellular Domain
- Measuring the Kinase Activity of GCKIII Proteins in vitro
- Spectrophotometric Method for Determining Glyoxalase 1 Activity in Cerebral Cavernous Malformation (CCM) Disease
- Fluorescence Analysis of Reactive Oxygen Species (ROS) in Cellular Models of Cerebral Cavernous Malformation Disease
- Small RNA Library Preparation for Profiling Small Non-coding RNAs in Patients Affected by Cavernous Malformations
- Affinity Purification and Preparation of Peptides for Mass Spectrometry from C. elegans. .